Processing biological samples for secondary electron (SE) sampling in the Scanning Electron Microscope (SEM) requires the removal of fluids from their matrix. This is typically done using Critical Point Drying (CPD) or Freeze Drying (FD). Hexamethyldisilazane (HMDS), a chemical with extremely low surface tension, is a great alternative approach to CPD or FD for SEM samples.

View the Kirsch Note here