Aug 21, 2018

SEM Biological HMDS Processing

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Edited: Aug 30, 2018

Processing biological samples for secondary electron (SE) sampling in the Scanning Electron Microscope (SEM) requires the removal of fluids from their matrix. This is typically done using Critical Point Drying (CPD) or Freeze Drying (FD). Hexamethyldisilazane (HMDS), a chemical with extremely low surface tension, is a great alternative approach to CPD or FD for SEM samples.

View the Kirsch Note here

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Michael Kostrna
Feb 15
SEM Specimen Mounting
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Michael Kostrna
Sep 10, 2018
Critical Point Drying (CPD) with the EMS Q850
Critical point drying is an established method of dehydrating biological tissue prior to examination in the Scanning Electron Microscope (SEM). Read the Kirsch Note here
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SEM Biological HMDS Processing

SEM Specimen Mounting

Critical Point Drying (CPD) with the EMS Q850

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