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I am currently working as an EM tech at a university. I am looking for a method or at least some direction on the prep of exosomes isolated from cultured cells.
My latest attempt was pelleting and embedding, however this did not work out as the exosomes need an ultra centrifuge to pellet and thus were crushed. I also tried whole mounting on formvar coated grids after fixation but we could not find intact samples. The two processes mentioned were from a manuscript (Jung).
If anyone has had experience in the prep and imaging of exosomes I would be most appreciative for your input.