Epoxy resins are the most commonly used embedment resins for routine ultrastructural transmission electron microscopy. The gold standard, Epon 812, is no longer produced but several similar resins such as Embed 812, Araldite, and Spurr’s have very good sectioning, contrast and beam stability, qualities that are required in a EM resin. Tissues that are to be used for immunocytochemical studies are typically embedded in acrylic resins such as LR White and Lowicryl, or Glyco-methacrylate a water miscible resin.
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When purchasing epoxy resins like Embed 812 or Araldite for electron microscopy, verify if VAT applies—some lab suppliers include it, while others list prices net. For international orders, precise VAT calculation prevents billing delays. A VAT calculator helps labs budget accurately, especially with specialized materials.
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Dear Michael,
I just got the question if UltraBed needs to be polimerized in absence of air. Could you please comment if so or possibly which resins at all need to be isolated from air during polimerisation.
Thanks a lot,
Stefan