There are 2 important qualities that a solvent used in the tissue processing protocol for TEM must have. Do you know what they are? Read the Kirsch Note here

Chemical fixation of biological samples for light and electron microscopies is required to halt biochemical reactions, prevent autolysis, stabilize macromolecules, preserve delicate morphologies and reduce artefacts which may be induced during other treatments such as dehydration. Besides these general objectives of all fixatives there are also some selected for performing specific functions, such as staining individual organelles or structures. Read on for a list of the more popular fixatives, their functions and other benefits. Read the Kirsch Note here

There are seven common buffers used for biological sample preparation for transmission electron microscopy (TEM): Phosphate (Sorensen's Phosphate, Millonig's Phosphate) Cacodylate, PHEM, S-Collidine, Tris, Hepes, and PIPES. There are three primary functions for the use of buffers in biological sample preparation. View the Kirsch Note here