This class will introduce participants to the use of equipment for processing tissue samples for TEM.
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Lecture, demonstration, and hands on operation of systems used. Discussions about advantages and disadvantages, along with participant questions, are a significant component of this workshop.
- EM sample preparation requirements / time table
- Microwave theory and processing
- Lynx II setup and operation
- Microscopy Innovations ASP 1000 setup and operation
- EMS 9000 Microwave
- Lynx II Processor
- Microscopy Innovations ASP 1000
- RMC Ultramicrotome
- Leica Ultramicrotome
- Zeiss TEM
Registration will be limited to a maximum of 15 participants.
EMS will provide samples to those who prefer not to bring their own.
Automated and Rapid Specimen Processing for Electron Microscopy
The preparation of samples for EM requires many steps, with extended wait times in between, requiring an entire day of a technician’s time. This process consists of several fixative, wash, dehydration, and resin infiltration steps, which are tedious and prone to temporal variability between runs.
With the use of an automated tissue processor or microwave, a technician’s time is reduced and better continuity between processing runs is obtained.
This class will introduce participants to the use of a dedicated lab microwave, EMS 9000, and 2 models of automated tissue processors Lynx II, and Microscopy Innovation’s ASP 1000 for processing tissue samples for TEM.