This course will give participants an overview of specimen preparation requirements.

Details

Targeted Participants

Individuals who are new to the field of microscopy with little or no experience with specimen processing, microtomy, or instrumentation.

Format

Lecture, demonstration, and hands-on practice, as well as round table tips and tricks discussions.

Main Curriculum

Specimen chemical preparation, theory and practical lab work, for OLM, TEM, and SEM
Sectioning for OLM (0.5 µm), TEM (100-60 nm)
Critical Point Drying (CPD) and HMDS prep for SEM
Instrument theory and hands on operation for OLM, TEM, and SEM

Instruments Available

Hitachi S3500 SEM
Leica UC7 Microtome
Zeiss TEM
Q150T Coater
RMC Power Tome XL
EMS 9000 Microwave
Lynx II Processor
K850 CPD
K750X Freeze Dryer
COXEM SEM EM-30N

Enrollment Note

Registration will be limited to a maximum of 15 participants.
EMS will provide samples to those who prefer not to bring their own.

Introduction to Microscopy Techniques

SKU: WS-R14

$1,200.00

This course will give participants an overview of the specimen preparation requirements, basic skill sets, and instrument theory/operation needed to perform as a technician in an electron microscopy laboratory.
Al Coritz
Al has been working in the Electron Microscopy field for 39 years, beginning at the Yale School of Medicine and ending up on the commercial side with several key EM companies. His specialty is Cryo-techniques and Thin Film Technology: i.e. Freeze Fracture/Rotary Shadowing, High Pressure Freezing, and more. He is currently with Electron Microscopy Sciences where he has been the Technical Director for over 20 years.

Michael Kostrna
Michael was the program director of the Electron Microscopy Technician program at Madison Area Technical College and has more than 35 years in EM technical education and research experience. He has been training EM students for 30 years and has developed curricula and lab exercises for TEM, SEM, OLM, lab safety, introductory and advanced biological EM, EM, maintenance, and x-Ray microanalysis. He has worked with companies such as SC Johnson Polymer, Dow Chemicals, Io Genetics, Virent Technologies, ABS Global, NanoOnocology, and Microscopy Inovations, and in the process gained insight to the various applications of EM.
Day 1
8:30-9:00 Introduction of staff and participants
9:00-10:30 Microscopes, overview of common systems
10:30-10:45 Coffee Break
10:45-12:00 Demo systems on OLM, TEM, and SEM
12:00-12:30 Provided lunch
12:30-1:30 OLM wet prep and Kohler alignment
1:30-2:30 Sectioning demo
2:30-4:30 Thick section + stain for OLM
6.00 Hosted Dinner

Day 2
8:30-9:00 Review of previous day’s activities
9:00-12:30 TEM chemical specimen prep (microwave) + Thin section
12:30-1:00 Provided lunch
1:00-2:00 TEM Lecture / demo
2:00-5:00 TEM hands on + sectioning (thick-thin)

Day 3
8:30-9:00 Review of previous day’s activities
9:00-10:30 SEM prep CPD - HMDS
10:30-10:45 Coffee Break
10:45-12:30 SEM theory lecture
12:30-1:00 Provided Lunch
1:00-2:00 SEM demo
2:00-4:30 SEM hands on
4:30-5:00 Round table discussion and work shop assessment

Schedule subject to change

Introduction to Microscopy Techniques

Scope of Class

Faculty

Schedule