Greetings I have been facing a problem with Technovit 9100. The polymerization never worked with me I have tried multiple times with the (stable and destabilized) basis solution in different conditions, however, I still end up with a liquid form which never hardens. Tissue type: Human acetabular bone with soft tissue attached. Total size: 13*10 mm.Human femoral head. Total size 5*5 mm. Protocol: 1) Dehydration on tissue rocker for (agitation): (50%, 70%, 80%, 95%, 95%) 1 hour each; (abs alcohol) overnight then (abs alcohol) for 1 hour. At room temperature 2) Intermedium on tissue rocker for (agitation): Xylin for (1 hour) then again Xylin for (1 hour). At room temperature 3) Pre-infiltration solution; Solution preparation: 100 ml of basis solution + ½ g Hardener 1. Solution used on tissue rocker for (agitation): 1 Hour At room temperature 4) Infiltration solution; Solution preparation: 100 ml of basis solution + 1.5g Hardener 1 + 10g of powder. Solution used for 24 Hour At 4C 5) Polymerization: Solution preparation: Stock A (100ml): 16g of powder + 80ml basis solution (mix until dissolve) + 0.8g Hardener 1 then top up the solution until 100 ml is reached. Stock B (100ml): 0.8 ml Hardener 2 + 0.4 ml Regulator + 100 ml basis solution Finally, the polymerization solution is used in a ratio of 9 parts from stock A + 1 part of stock B mixed immediately before use. We have tried a number of conditions to see if the polymerization will happen: Solution with the tissue at (RT, 4C and - 15C) for 24HSolution with the tissue at (RT, 4C and - 15C) for 72HSolution without the tissue at (RT, 4C and - 15C) for 24H However, all of that ended unsuccessfully. I would really appreciate any insight or help to this matter Sincerely Abdulaziz

When working with solutions for biological applications, distilled or deionized water is usually the solvent of choice. View the Kirsch Note here